Early events in Populus hybrid and Fagus sylvatica leaves exposed to ozone.
Identifieur interne : 003299 ( Main/Exploration ); précédent : 003298; suivant : 003300Early events in Populus hybrid and Fagus sylvatica leaves exposed to ozone.
Auteurs : R. Desotgiu ; F. Bussotti ; F. Faoro ; M. Iriti ; G. Agati ; R. Marzuoli ; G. Gerosa ; C. TaniSource :
- TheScientificWorldJournal [ 1537-744X ] ; 2010.
Descripteurs français
- KwdFr :
- Chlorophylle (métabolisme), Chlorophylle A (MeSH), Cinétique (MeSH), Fagus (métabolisme), Fagus (physiologie), Fagus (ultrastructure), Feuilles de plante (métabolisme), Feuilles de plante (physiologie), Feuilles de plante (ultrastructure), Microscopie électronique à transmission (MeSH), Ozone (MeSH), Peroxyde d'hydrogène (métabolisme), Populus (métabolisme), Populus (physiologie), Populus (ultrastructure), Spectrométrie de fluorescence (MeSH).
- MESH :
- métabolisme : Chlorophylle, Fagus, Feuilles de plante, Peroxyde d'hydrogène, Populus.
- physiologie : Fagus, Feuilles de plante, Populus.
- Chlorophylle A, Cinétique, Fagus, Feuilles de plante, Microscopie électronique à transmission, Ozone, Populus, Spectrométrie de fluorescence.
English descriptors
- KwdEn :
- Chlorophyll (metabolism), Chlorophyll A (MeSH), Fagus (metabolism), Fagus (physiology), Fagus (ultrastructure), Hydrogen Peroxide (metabolism), Kinetics (MeSH), Microscopy, Electron, Transmission (MeSH), Ozone (MeSH), Plant Leaves (metabolism), Plant Leaves (physiology), Plant Leaves (ultrastructure), Populus (metabolism), Populus (physiology), Populus (ultrastructure), Spectrometry, Fluorescence (MeSH).
- MESH :
- chemical , metabolism : Chlorophyll, Hydrogen Peroxide.
- chemical : Chlorophyll A, Ozone.
- metabolism : Fagus, Plant Leaves, Populus.
- physiology : Fagus, Plant Leaves, Populus.
- ultrastructure : Fagus, Plant Leaves, Populus.
- Kinetics, Microscopy, Electron, Transmission, Spectrometry, Fluorescence.
Abstract
This paper aims to investigate early responses to ozone in leaves of Fagus sylvatica (beech) and Populus maximowiczii x Populus berolinensis (poplar). The experimental setup consisted of four open-air (OA) plots, four charcoal-filtered (CF) open-top chambers (OTCs), and four nonfiltered (NF) OTCs. Qualitative and quantitative analyses were carried out on nonsymptomatic (CF) and symptomatic (NF and OA) leaves of both species. Qualitative analyses were performed applying microscopic techniques: Evans blue staining for detection of cell viability, CeCl3 staining of transmission electron microscope (TEM) samples to detect the accumulation of H2O2, and multispectral fluorescence microimaging and microspectrofluorometry to investigate the accumulation of fluorescent phenolic compounds in the walls of the damaged cells. Quantitative analyses consisted of the analysis of the chlorophyll a fluorescence transients (fast kinetics). The early responses to ozone were demonstrated by the Evans blue and CeCl3 staining techniques that provided evidence of plant responses in both species 1 month before foliar symptoms became visible. The fluorescence transients analysis, too, demonstrated the breakdown of the oxygen evolving system and the inactivation of the end receptors of electrons at a very early stage, both in poplar and in beech. The accumulation of phenolic compounds in the cell walls, on the other hand, was a species-specific response detected in poplar, but not in beech. Evans blue and CeCl3 staining, as well as the multispectral fluorescence microimaging and microspectrofluorometry, can be used to support the field diagnosis of ozone injury, whereas the fast kinetics of chlorophyll fluorescence provides evidence of early physiological responses.
DOI: 10.1100/tsw.2010.63
PubMed: 20364237
PubMed Central: PMC5763675
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Early events in Populus hybrid and Fagus sylvatica leaves exposed to ozone.</title><author><name sortKey="Desotgiu, R" sort="Desotgiu, R" uniqKey="Desotgiu R" first="R" last="Desotgiu">R. Desotgiu</name><affiliation><nlm:affiliation>Dipartimento di Biotecnologie Agrarie, Sezione di Botanica Applicata e Ambientale, Università di Firenze.</nlm:affiliation><wicri:noCountry code="subField">Università di Firenze</wicri:noCountry></affiliation></author><author><name sortKey="Bussotti, F" sort="Bussotti, F" uniqKey="Bussotti F" first="F" last="Bussotti">F. Bussotti</name></author><author><name sortKey="Faoro, F" sort="Faoro, F" uniqKey="Faoro F" first="F" last="Faoro">F. Faoro</name></author><author><name sortKey="Iriti, M" sort="Iriti, M" uniqKey="Iriti M" first="M" last="Iriti">M. Iriti</name></author><author><name sortKey="Agati, G" sort="Agati, G" uniqKey="Agati G" first="G" last="Agati">G. Agati</name></author><author><name sortKey="Marzuoli, R" sort="Marzuoli, R" uniqKey="Marzuoli R" first="R" last="Marzuoli">R. Marzuoli</name></author><author><name sortKey="Gerosa, G" sort="Gerosa, G" uniqKey="Gerosa G" first="G" last="Gerosa">G. Gerosa</name></author><author><name sortKey="Tani, C" sort="Tani, C" uniqKey="Tani C" first="C" last="Tani">C. Tani</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20364237</idno><idno type="pmid">20364237</idno><idno type="doi">10.1100/tsw.2010.63</idno><idno type="pmc">PMC5763675</idno><idno type="wicri:Area/Main/Corpus">003239</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003239</idno><idno type="wicri:Area/Main/Curation">003239</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003239</idno><idno type="wicri:Area/Main/Exploration">003239</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Early events in Populus hybrid and Fagus sylvatica leaves exposed to ozone.</title><author><name sortKey="Desotgiu, R" sort="Desotgiu, R" uniqKey="Desotgiu R" first="R" last="Desotgiu">R. Desotgiu</name><affiliation><nlm:affiliation>Dipartimento di Biotecnologie Agrarie, Sezione di Botanica Applicata e Ambientale, Università di Firenze.</nlm:affiliation><wicri:noCountry code="subField">Università di Firenze</wicri:noCountry></affiliation></author><author><name sortKey="Bussotti, F" sort="Bussotti, F" uniqKey="Bussotti F" first="F" last="Bussotti">F. Bussotti</name></author><author><name sortKey="Faoro, F" sort="Faoro, F" uniqKey="Faoro F" first="F" last="Faoro">F. Faoro</name></author><author><name sortKey="Iriti, M" sort="Iriti, M" uniqKey="Iriti M" first="M" last="Iriti">M. Iriti</name></author><author><name sortKey="Agati, G" sort="Agati, G" uniqKey="Agati G" first="G" last="Agati">G. Agati</name></author><author><name sortKey="Marzuoli, R" sort="Marzuoli, R" uniqKey="Marzuoli R" first="R" last="Marzuoli">R. Marzuoli</name></author><author><name sortKey="Gerosa, G" sort="Gerosa, G" uniqKey="Gerosa G" first="G" last="Gerosa">G. Gerosa</name></author><author><name sortKey="Tani, C" sort="Tani, C" uniqKey="Tani C" first="C" last="Tani">C. Tani</name></author></analytic><series><title level="j">TheScientificWorldJournal</title><idno type="eISSN">1537-744X</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chlorophyll (metabolism)</term><term>Chlorophyll A (MeSH)</term><term>Fagus (metabolism)</term><term>Fagus (physiology)</term><term>Fagus (ultrastructure)</term><term>Hydrogen Peroxide (metabolism)</term><term>Kinetics (MeSH)</term><term>Microscopy, Electron, Transmission (MeSH)</term><term>Ozone (MeSH)</term><term>Plant Leaves (metabolism)</term><term>Plant Leaves (physiology)</term><term>Plant Leaves (ultrastructure)</term><term>Populus (metabolism)</term><term>Populus (physiology)</term><term>Populus (ultrastructure)</term><term>Spectrometry, Fluorescence (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Chlorophylle (métabolisme)</term><term>Chlorophylle A (MeSH)</term><term>Cinétique (MeSH)</term><term>Fagus (métabolisme)</term><term>Fagus (physiologie)</term><term>Fagus (ultrastructure)</term><term>Feuilles de plante (métabolisme)</term><term>Feuilles de plante (physiologie)</term><term>Feuilles de plante (ultrastructure)</term><term>Microscopie électronique à transmission (MeSH)</term><term>Ozone (MeSH)</term><term>Peroxyde d'hydrogène (métabolisme)</term><term>Populus (métabolisme)</term><term>Populus (physiologie)</term><term>Populus (ultrastructure)</term><term>Spectrométrie de fluorescence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chlorophyll</term><term>Hydrogen Peroxide</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Chlorophyll A</term><term>Ozone</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fagus</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chlorophylle</term><term>Fagus</term><term>Feuilles de plante</term><term>Peroxyde d'hydrogène</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Fagus</term><term>Feuilles de plante</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fagus</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Fagus</term><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Kinetics</term><term>Microscopy, Electron, Transmission</term><term>Spectrometry, Fluorescence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chlorophylle A</term><term>Cinétique</term><term>Fagus</term><term>Feuilles de plante</term><term>Microscopie électronique à transmission</term><term>Ozone</term><term>Populus</term><term>Spectrométrie de fluorescence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This paper aims to investigate early responses to ozone in leaves of Fagus sylvatica (beech) and Populus maximowiczii x Populus berolinensis (poplar). The experimental setup consisted of four open-air (OA) plots, four charcoal-filtered (CF) open-top chambers (OTCs), and four nonfiltered (NF) OTCs. Qualitative and quantitative analyses were carried out on nonsymptomatic (CF) and symptomatic (NF and OA) leaves of both species. Qualitative analyses were performed applying microscopic techniques: Evans blue staining for detection of cell viability, CeCl3 staining of transmission electron microscope (TEM) samples to detect the accumulation of H2O2, and multispectral fluorescence microimaging and microspectrofluorometry to investigate the accumulation of fluorescent phenolic compounds in the walls of the damaged cells. Quantitative analyses consisted of the analysis of the chlorophyll a fluorescence transients (fast kinetics). The early responses to ozone were demonstrated by the Evans blue and CeCl3 staining techniques that provided evidence of plant responses in both species 1 month before foliar symptoms became visible. The fluorescence transients analysis, too, demonstrated the breakdown of the oxygen evolving system and the inactivation of the end receptors of electrons at a very early stage, both in poplar and in beech. The accumulation of phenolic compounds in the cell walls, on the other hand, was a species-specific response detected in poplar, but not in beech. Evans blue and CeCl3 staining, as well as the multispectral fluorescence microimaging and microspectrofluorometry, can be used to support the field diagnosis of ozone injury, whereas the fast kinetics of chlorophyll fluorescence provides evidence of early physiological responses.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20364237</PMID><DateCompleted><Year>2010</Year><Month>06</Month><Day>24</Day></DateCompleted><DateRevised><Year>2018</Year><Month>12</Month><Day>01</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1537-744X</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><PubDate><Year>2010</Year><Month>Apr</Month><Day>01</Day></PubDate></JournalIssue><Title>TheScientificWorldJournal</Title><ISOAbbreviation>ScientificWorldJournal</ISOAbbreviation></Journal><ArticleTitle>Early events in Populus hybrid and Fagus sylvatica leaves exposed to ozone.</ArticleTitle><Pagination><MedlinePgn>512-27</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1100/tsw.2010.63</ELocationID><Abstract><AbstractText>This paper aims to investigate early responses to ozone in leaves of Fagus sylvatica (beech) and Populus maximowiczii x Populus berolinensis (poplar). The experimental setup consisted of four open-air (OA) plots, four charcoal-filtered (CF) open-top chambers (OTCs), and four nonfiltered (NF) OTCs. Qualitative and quantitative analyses were carried out on nonsymptomatic (CF) and symptomatic (NF and OA) leaves of both species. Qualitative analyses were performed applying microscopic techniques: Evans blue staining for detection of cell viability, CeCl3 staining of transmission electron microscope (TEM) samples to detect the accumulation of H2O2, and multispectral fluorescence microimaging and microspectrofluorometry to investigate the accumulation of fluorescent phenolic compounds in the walls of the damaged cells. Quantitative analyses consisted of the analysis of the chlorophyll a fluorescence transients (fast kinetics). The early responses to ozone were demonstrated by the Evans blue and CeCl3 staining techniques that provided evidence of plant responses in both species 1 month before foliar symptoms became visible. The fluorescence transients analysis, too, demonstrated the breakdown of the oxygen evolving system and the inactivation of the end receptors of electrons at a very early stage, both in poplar and in beech. The accumulation of phenolic compounds in the cell walls, on the other hand, was a species-specific response detected in poplar, but not in beech. Evans blue and CeCl3 staining, as well as the multispectral fluorescence microimaging and microspectrofluorometry, can be used to support the field diagnosis of ozone injury, whereas the fast kinetics of chlorophyll fluorescence provides evidence of early physiological responses.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Desotgiu</LastName><ForeName>R</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Dipartimento di Biotecnologie Agrarie, Sezione di Botanica Applicata e Ambientale, Università di Firenze.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bussotti</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Faoro</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Iriti</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Agati</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Marzuoli</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Gerosa</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Tani</LastName><ForeName>C</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>04</Month><Day>01</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>ScientificWorldJournal</MedlineTA><NlmUniqueID>101131163</NlmUniqueID><ISSNLinking>1537-744X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>1406-65-1</RegistryNumber><NameOfSubstance UI="D002734">Chlorophyll</NameOfSubstance></Chemical><Chemical><RegistryNumber>66H7ZZK23N</RegistryNumber><NameOfSubstance UI="D010126">Ozone</NameOfSubstance></Chemical><Chemical><RegistryNumber>BBX060AN9V</RegistryNumber><NameOfSubstance UI="D006861">Hydrogen Peroxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>YF5Q9EJC8Y</RegistryNumber><NameOfSubstance UI="D000077194">Chlorophyll A</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002734" MajorTopicYN="N">Chlorophyll</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000077194" MajorTopicYN="N">Chlorophyll A</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D029964" MajorTopicYN="N">Fagus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006861" MajorTopicYN="N">Hydrogen Peroxide</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D046529" MajorTopicYN="N">Microscopy, Electron, Transmission</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010126" MajorTopicYN="Y">Ozone</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013050" MajorTopicYN="N">Spectrometry, Fluorescence</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>4</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>4</Month><Day>7</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>6</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20364237</ArticleId><ArticleId IdType="doi">10.1100/tsw.2010.63</ArticleId><ArticleId IdType="pmc">PMC5763675</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Agati, G" sort="Agati, G" uniqKey="Agati G" first="G" last="Agati">G. Agati</name><name sortKey="Bussotti, F" sort="Bussotti, F" uniqKey="Bussotti F" first="F" last="Bussotti">F. Bussotti</name><name sortKey="Desotgiu, R" sort="Desotgiu, R" uniqKey="Desotgiu R" first="R" last="Desotgiu">R. Desotgiu</name><name sortKey="Faoro, F" sort="Faoro, F" uniqKey="Faoro F" first="F" last="Faoro">F. Faoro</name><name sortKey="Gerosa, G" sort="Gerosa, G" uniqKey="Gerosa G" first="G" last="Gerosa">G. Gerosa</name><name sortKey="Iriti, M" sort="Iriti, M" uniqKey="Iriti M" first="M" last="Iriti">M. Iriti</name><name sortKey="Marzuoli, R" sort="Marzuoli, R" uniqKey="Marzuoli R" first="R" last="Marzuoli">R. Marzuoli</name><name sortKey="Tani, C" sort="Tani, C" uniqKey="Tani C" first="C" last="Tani">C. Tani</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003299 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003299 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:20364237
|texte= Early events in Populus hybrid and Fagus sylvatica leaves exposed to ozone.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:20364237" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |